Single-molecule direct RNA sequencing without cDNA synthesis

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Single molecule sequencing of the M13 virus genome without amplification

Next generation sequencing (NGS) has revolutionized life sciences research. However, GC bias and costly, time-intensive library preparation make NGS an ill fit for increasing sequencing demands in the clinic. A new class of third-generation sequencing platforms has arrived to meet this need, capable of directly measuring DNA and RNA sequences at the single-molecule level without amplification. ...

متن کامل

Single-molecule, motion-based DNA sequencing using RNA polymerase.

We present a method for sequencing DNA that relies on the motion of single RNA polymerase molecules. When a given nucleotide species limits the rate of transcription, polymerase molecules pause at positions corresponding to the rare base. An ultrastable optical trapping apparatus capable of base pair resolution was used to monitor transcription under limiting amounts of each of the four nucleot...

متن کامل

Single Molecule Fluorescence Microscopy and its Applications to Single Molecule Sequencing by Cyclic Synthesis

1.0. Introduction 2.0. Background 2.1. Single Molecule Detection 2.2. Total Internal Reflection 2.3. FRET Theory 3.0. DNA Sequencing by Cyclic synthesis 3.1. Motivation 3.2. Surface Treatment 3.3. Polymerase Kinetics 3.4. Sequencing Strategies 3.4.1. Cyclic Synthesis using FRET 3.4.2. Real Time Imaging 3.4.3. Non FRET Imaging 3.4.4. Cleavable Linkers 3.4.5. Cleavable Terminators 3.4.6. Multi-Co...

متن کامل

RNA editing of wheat mitochondrial ATP synthase subunit 9: direct protein and cDNA sequencing.

RNA editing of subunit 9 of the wheat mitochondrial ATP synthase has been studied by cDNA and protein sequence analysis. Most of the cDNA clones sequenced (95%) showed that editing by C-to-U transitions occurred at eight positions in the coding region. Consequently, 5 amino acids were changed in the protein when compared with the sequence predicted from the gene. Two edited codons gave no chang...

متن کامل

RNA-sequencing from single nuclei.

It has recently been established that synthesis of double-stranded cDNA can be done from a single cell for use in DNA sequencing. Global gene expression can be quantified from the number of reads mapping to each gene, and mutations and mRNA splicing variants determined from the sequence reads. Here we demonstrate that this method of transcriptomic analysis can be done using the extremely low le...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Wiley Interdisciplinary Reviews: RNA

سال: 2011

ISSN: 1757-7004

DOI: 10.1002/wrna.84